Glipizide microspheres containing chitosan were prepared by simple emulsification phase separation technique using glutaraldehyde as a crosslinking agent. In future microspheres will find the central place in novel drug delivery, particularly in diseased cell sorting, diagnostics, genetic materials. The measured weight was divided by the total amount of drug and polymers which were used for the preparation of the microspheres to obtained percentage yield. The purpose of this research was to formulate and systematically evaluate in vitro and in vivo performances of mucoadhesive microspheres of glipizide. Microspheres are characteristically free flowing powders consisting of proteins or synthetic polymers which are biodegradable in nature and ideally having a particle size less than 200. References powders and granulates freeflowing powders and granulates are needed for a variety of industrial processes. Plga microspheres containing fluorescein isothiocyanate labeled bovine serum albumin and horseradish peroxidase were prepared by a modified solvent evaporation method using a double emulsion of the. Syed ershad, v sai kishore, u kartheek, m sandeep, k prameela rani, and k adithya. International journal of pharmaceutical sciences and research. Microspheres may provide more prolonged contact with the nasal mucosa, which enhances rate and extent of drug absorption 115. Application and advancement of microsphere as controlled delivery system. Pdf the concept of targeted drug delivery is designed for attempting to concentrate the drug in the tissues of interest while reducing the. Formulation and evaluation of microspheres emulsion. Microsphere formulations using eudragit s100 as a carrier polymer were prepared using emulsion solvent evaporation technique.
Research article open access formulation and evaluation of. Process for preparation of microcapsules and microspheres 67. Phase separation is then accomplished by changing the solution conditions by the salt addition, onsolvent addition, addition of the incompatible polymer or change in ph. Octoberdecember 20 research article ijpsn322rajkumar. Solid and hollow microspheres vary widely in density and, therefore, are used for different applications. These microspheres were freeze dried over night and kept in an airtight container for further studies paharia et al.
Sep 30, 2014 formulation and evaluation of microspheres 1. Interfacial polymerization technique when two reactive monomers are. Zidovudine is the first approved compound for the treatment of hiv. Preparation and characterization of microspheres for. Microspheres and microcapsules, a survey of manufacturing. Formulation and evaluation of microspheres based on gelatin. Further, the process of targeting and site specific delivery with absolute accuracy can be achieved by attaching bioactive molecule to liposome, bioerodable polymer, implants, monoclonal antibodies and various particulate carriers e. Preparation and evaluation of alginate microspheres of piroxicam for. Preparation and evaluation sustainedrelease injectable microspheres 379 journal of microencapsulation downloaded from by monash university on 0502 for personal use only.
Research paper preparation and characterization of. The nicorandilloaded chitosan microspheres were formulated by emulsion crosslinking method. The microspheres showed similar release profile as compared to the marketed sample. To prepare and evaluate metformin microspheres for prolonged release. Khamanga, natalie parfitt,tsitsi nyamuzhiwa, hendrina haidula, and roderick b. Deshmukh t, deshmukh v, jadhav p, kasat k and patil r. V prasanth, akashmoy chakraborty, sam t mathew, rinku mathappan, and v. Microspheres microspheres are small spherical particles, with diameter 1. Preparation and evaluation of hollow microsphere drug delivery system of zidovudine abstract. Formulation and evaluation of microspheres of serratiopeptidase the known amount of drug serratiopeptidase was mixed with chitosan solution in the preparation of microspheres as per method reported by thanoo et al. Hollow microspheres are typically used as additives to lower the density of a material. The present investigation was carried out on the formulation and evaluation of oral controlled release microspheres of repaglinide, which is meant for treatment of type ii diabetes mellitus.
Blue dextran was used as a watersoluble biomacromolecular drug model. Formulation and evaluation of coated microspheres for colon. Further, the process of targeting and site specific delivery with absolute accuracy can be achieved by attaching bioactive molecule to liposome, bioerodable polymer, implants, monoclonal antibodies and. The collected microspheres were dried in desiccators over night. The microspheres were discrete, spherical and uniform in shape. Preparation of polysaccharidebased microspheres by a water. They are lightweight, chemically inert, and mechanically strong. Individual core particles coated with a film of shell formation are flung off the. Preparation of microspheres by the solvent evaporation technique. Polyurethene, ureaformaldehyde, pmma, polystyrene 10 15 20 ncy % 0.
Synthesis and evaluation of expancel microspheres replacement of a chemical of environmental concern tomas gardfors degree project in engineering chemistry, 30 hp. Development and evaluation of floating microspheres of curcumin kapil kumar1 and ak rai2 1institute of pharmacy nims university, jaipur, 303121, rajasthan, global institute of pharmaceutical education and research, kashipur, u. However, their toxicityirritancy on the nasal mucosa due to the. Results of preliminary trials indicate that volume of crosslinking agent, time for. Microparticles, microspheres, and microcapsules for. Formulation development and evaluation of alginate. Formulation and evaluation of salbutamol sulphate microspheres by solvent evaporation method v.
We show that spectroscopy of such chromophores can be done by adsorption to. Preparation and evaluation of trimetazidine hydrochloride microspheres using chitosan basu s. After 24 hr the swollen microspheres were taken out. Preparation and evaluation of bioadhesive microspheres prepared by. Preparation and invitro evaluation of metformin microspheres using nonaqueous solvent evaporation technique navneet garud and akanksha garud institute of professional studies college of pharmacy, shivpuri link road, gwalior mp, india. Preparation and evaluation of floating microspheres of. Department of pharmaceutics, bapatla college of pharmacy, bapatla, guntur dist 522101, andhra pradesh, india. Preparation and invitro evaluation of metformin microspheres. The microspheres were stored at 4 until the time of evaluation. Microspheres methods for preparation of microspheres. A well designed controlled drug delivery system can overcome some of the problems of conventional therapy and enhance the therapeutic efficacy of a given drug. Evaluation of microspheres as surrogates for cryptosporidium. The percentage yield of microspheres was determined from the ratio of solidified total microspheres to the solid material used in the inner phase multiplied by 100.
Solvent evaporation and ionic gelation methods are employed for preparing microspheres. Density marker beads are small colored microspheres of known mass density that are used for calibrating density gradients and determining density in gradient columns. Preparation and evaluation of sustained release loxoprofen loaded. Microspheres are considered to be one of the most promising floating systems, because they combine the advantages of multiple unit systems, good floating properties and are prepared using assorted polymers curatolo et al. Development and evaluation of floating microspheres of curcumin.
Microspheres can be taken up from the intestine into the immuneinductive environment of the peyers patches, where they can induce both mucosal and systemic immune responses. The range of techniques for the preparation of microspheres provides multiple options to control as drug administration aspects and to enhance the therapeutic efficacy of a given the drug. Formulation and evaluation of microspheres of serratiopeptidase the known amount of drug serratiopeptidase was mixed with chitosan solution in the preparation of microspheres as per. Evaluation of microspheres percentage yield the prepared microspheres were collected and weighed from different formulations. Microspheres are characteristically free flowing powders having particle size ranging from 1.
We evaporated the aqueous buffer from the microspheres and resuspended the microspheres inside silicone oil. Preparation of gelatin microspheres loaded with diclofenac sodium as given in the table. However, the success of these microspheres is limited owing to their short residence time at the site of absorption. Preparation of a wide range of microspherical and microcapsular products based on biodegradable polyesters, polysaccharides, and nonbiodegradable polymers are discussed. Preparation and evaluation of floating microspheres of ritonavir. The percentage of microspheres was calculated by the following equation %floating microspheres weight of floating microspheres initial weight of floating microspheres. Microspheres are made from polymeric, waxy or protective materials that is biodegradable synthetic polymers and modified natural products. The term nanospheres is often applied to the smaller spheres sized 10 to 500 nm to distinguish them from larger microspheres microbeads and beads are used alternatively for. Application of microspheres in pharmaceutical industry. Polymer ratio and volume of glutaraldehyde ga on percentage entrapment efficiency, particle size, and % drug release at 8 h. These, however, do not always meet the exacting standards which modern manufacturing. Preparation and evaluation of trimetazidine hydrochloride. Phase separation is then accomplished by changing the.
Polysaccharidebased microspheres of chitosan, starch, and alginate were prepared by the waterinoil emulsion solvent diffusion method for use as drug carriers. Plga microspheres containing fluorescein isothiocyanate labeled bovine serum albumin and horseradish peroxidase were prepared by a modified solvent evaporation method using a double emulsion of the wow type. Microspheres developed using polymer exhibits favourable biological behaviour such as bioadhesion, permeabilityenhancing properties, and. Kamalakkannan salbutamol sulphate ss loaded microspheres were prepared by solvent evaporation. Application and advancement of microsphere as controlled. A 3 2 factorial design was employed to study the influence of drug. Pdf an overview of preparation and evaluation sustained. Walker1 faculty of pharmacy, rhodes university, grahamstown, south africa 6140 abstract.
Polymethyl methacrylate or pmma is less hydrophobic than polystyrene and should show reduced nonspecific protein and peptide binding. The evaluation of eudragit microcapsules manufactured by. Microspheres constitute an important part of novel drug delivery system by virtue of their small size and efficient carrier capacity. The evaluation of eudragit microcapsules manufactured by solvent evaporation using usp apparatus 1 sandile m. Preparation of microspheres by the solvent evaporation.
The prepared albumin microspheres released the drug completely within 10 hours at lower drug to polymer ratio. Glibenclamide microspheres were developed by ionotropic gelation. Introduction definition of microspheres microparticles or microspheres are defined as small spheres made of any material and sized from about 50 nm to about 2 mm. Pdf preparation and evaluation of valacyclovir microspheres. Formulation and evaluation of mucoadhesive glipizide microspheres. Preparation and evaluation of chitosan microspheres. Microspheres are characteristically free flowing powders consisting of proteins or synthetic polymers having a. The yield of microspheres and encapsulation efficiencies were high for all the formulations and were not affected by the type of polymer and drug polymer ratio and stirring speed. The microspheres were spherical with diameters ranging form 55 to 95. Microspheres for intranasal applications are usually prepared using. Development and characterization of polymeric microspheres. Hollow phenolic microspheres are excellent for use in a variety of industrial applications.
A well designed controlled drug delivery system can overcome. Microspheres for intranasal applications are usually prepared using biocompatible materials, such as starch, albumin, dextran, and gelatin 33,77. The effect of the following formulation and process variables on the. Preparation and evaluation of floating risedronate sodium.
A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. The aim of present study was to formulate and evaluate the loxoprofen loaded sustained release microspheres by emulsion. Interfacial polymerization technique when two reactive monomers are dissolved in immiscible solvents, the monomers diffuse to the oil water interface where they react to form a polymeric membrane that envelopes dispersed phase. Development of microspheres for biomedical applications. Microspheres, advancement, applications, preparation, evaluation parameters introduction previously people have. Formulation and evaluation of mucoadhesive microspheres of ziprasidone hydrochloride for oral controlled release. May 29, 2016 microspheres methods for preparation of microspheres 1. Content definition history material used criteria for selection of method methods of preparation applications references 2sagar savale. Contents of the powerpoint on formulation and evaluation of microspheres include. Specified quantity of diclofenac sodium was dissolved separately in 3 ml of phosphate buffer ph 7. Jan 22, 2014 contents of the powerpoint on formulation and evaluation of microspheres include. Microspheres received much attention not only for prolonged release, but also for targeting of drugs. The invitro releases of the drug from microspheres were studied at ph 1. The procedure was repeated to obtain smucin to gelatin in ratios of.
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